| 质粒名称: | pcDNA3 Flag-humanTRAF6 (1093) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 66929 |
| 存储实验室: | Michael Karin |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TRAF6 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | Flag（插入N端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3 Flag-humanTRAF6 (1093) was a gift from Michael Karin (Addgene plasmid # 66929 ; http://n2t.net/addgene:66929 ; RRID:Addgene_66929) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pcDNA3 Flag-humanTRAF6 (1093) 6974 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Mammalian Expression of TRAF6.
ACCESSION .
VERSION .
KEYWORDS pcDNA3 Flag-humanTRAF6 (1093)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6974)
TITLE Karin Lab plasmids
REFERENCE 2 (bases 1 to 6974)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6974
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(54..73)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 245..624
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 625..828
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 779..799
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 873..892
/label=T7
/note="T7 promoter, forward primer"
promoter 873..891
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 911..934
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
promoter complement(2537..2555)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2538..2555)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(2575..2592)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2581..2805
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2851..3279
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2938..2957)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3148..3169
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3288..3308)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3293..3622
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3473..3608
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3535..3554
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3689..4483
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3743..3762)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4353..4372
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4657..4778
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4694..4713)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4748..4767
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4827..4843)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4827..4843)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4840..4862)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4851..4867
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4875..4905)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4920..4941
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5058..5075)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5229..5817)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5309..5328)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5988..6848)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6611..6630
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6849..6953)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
61 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
121 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
181 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
241 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
301 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
841 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
901 gcttaccatg gactacaagg atgacgatga caaggaattc atgagtctgc taaactgtga
961 aaacagctgt ggatccagcc agtctgaaag tgactgctgt gtggccatgg ccagctcctg
1021 tagcgctgta acaaaagatg atagtgtggg tggaactgcc agcacgggga acctctccag
1081 ctcatttatg gaggagatcc agggatatga tgtagagttt gacccacccc tggaaagcaa
1141 gtatgaatgc cccatctgct tgatggcatt acgagaagca gtgcaaacgc catgcggcca
1201 taggttctgc aaagcctgca tcataaaatc aataagggat gcaggtcaca aatgtccagt
1261 tgacaatgaa atactgctgg aaaatcaact atttccagac aattttgcaa aacgtgagat
1321 tctttctctg atggtgaaat gtccaaatga aggttgtttg cacaagatgg aactgagaca
1381 tcttgaggat catcaagcac attgtgagtt tgctcttatg gattgtcccc aatgccagcg
1441 tcccttccaa aaattccata ttaatattca cattctgaag gattgtccaa ggagacaggt
1501 ttcttgtgac aactgtgctg catcaatggc atttgaagat aaagagatcc atgaccagaa
1561 ctgtcctttg gcaaatgtca tctgtgaata ctgcaatact atactcatca gagaacagat
1621 gcctaatcat tatgatctag actgccctac agccccaatt ccatgcacat tcagtacttt
1681 tggttgccat gaaaagatgc agaggaatca cttggcacgc cacctacaag agaacaccca
1741 gtcacacatg agaatgttgg cccaggctgt tcatagtttg agcgttatac ccgactctgg
1801 gtatatctca gaggtccgga atttccagga aactattcac cagttagagg gtcgccttgt
1861 aagacaagac catcaaatcc gggagctgac tgctaaaatg gaaactcaga gtatgtatgt
1921 aagtgagctc aaacgaacca ttcgaaccct tgaggacaaa gttgctgaaa tcgaagcaca
1981 gcagtgcaat ggaatttata tttggaagat tggcaacttt ggaatgcatt tgaaatgtca
2041 agaagaggag aaacctgttg tgattcatag ccctggattc tacactggca aacccgggta
2101 caaactgtgc atgcgcttgc accttcagtt accgactgct cagcgctgtg caaactatat
2161 atcccttttt gtccacacaa tgcaaggaga atatgacagc cacctccctt ggcccttcca
2221 gggtacaata cgccttacaa ttcttgatca gtctgaagca cctgtaaggc aaaaccacga
2281 agagataatg gatgccaaac cagagctgct tgctttccag cgacccacaa tcccacggaa
2341 cccaaaaggt tttggctatg taacttttat gcatctggaa gccctaagac aaagaacttt
2401 cattaaggat gacacattat tagtgcgctg tgaggtctcc acccgctttg acatgggtag
2461 ccttcggagg gagggttttc agccacgaag tactgatgca ggggtatagc tcgagcatgc
2521 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
2581 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
2641 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
2701 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
2761 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
2821 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
2881 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
2941 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
3001 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
3061 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
3121 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
3181 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
3241 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
3301 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
3361 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
3421 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
3481 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
3541 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
3601 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
3661 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
3721 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
3781 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
3841 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
3901 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
3961 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
4021 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
4081 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
4141 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
4201 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
4261 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
4321 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
4381 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
4441 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
4501 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
4561 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
4621 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
4681 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
4741 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
4801 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
4861 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
4921 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
4981 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
5041 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
5101 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
5161 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
5221 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
5281 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
5341 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
5401 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
5461 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
5521 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
5581 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
5641 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
5701 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
5761 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
5821 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
5881 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
5941 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
6001 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
6061 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
6121 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
6181 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
6241 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
6301 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
6361 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
6421 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
6481 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
6541 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
6601 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
6661 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
6721 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
6781 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
6841 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
6901 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
6961 ccccgaaaag tgcc
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pEP4 E02S EN2K
- 下一篇：pEBB HA RelA
